Research Interests

1) Gene delivery and gene therapy.  

Vector development and improvement of gene transfer techniques by enhancing DNA-nuclear delivery.  Enhancement of non-viral delivery of large DNA constructs for gene therapy using ultrasound.

Gene delivery to the mitochondria. Construct development and direct delivery of DNA to mitochondria using peptide conjugates of mitochondriotropic complexes. (See) 

2) Viral Genetics of the Human Cytomegalovirus (HCMV).  

Genetic analysis of HCMV using Bacteria Artificial Chromosome (BAC) system. 

UNDERGRADUATE TEACHING

Lecturer

• GEN 31HMG (Human Molecular genetics)
• Medical Sciences 31MCG ( Human Genome & Genetic diseases)
• BIO 12GEN (Genetics, Human Biology & Evolution)
• GEN 21 HMG (Human and Molecular genetics) 

Selected Publications

1.       DA Jans, CK Chan & S Hubner (1998). Nuclear localization signals and the regulation of their activity by phosphorylation: application in drug delivery to the nucleus. Medicinal Research Reviews. 18 (4) 189-223.

2.      CK Chan, S Hubner, W Hu and DA Jans (1998). Mutual exclusivity of DNA binding and nuclear localization signal recognition by the yeast transcription factor GAL4: implications for nonviral DNA delivery. Gene Therapy, Sep;5(9):1204-12.

3.      CK Chan and DA Jans (1999). Enhancement of polylysine-mediated transfection by nuclear localization sequences (NLSs): polylysine does not function as an NLS. Human Gene Therapy, Jul 1;10(10):1695-702

4.      S Hubner, HMS Smith, W Hu, CK Chan, HP Rihs, BM Paschal, NV Raikhel and DA Jans (1999). Plant importin a binds nuclear localization sequences with high affinity and can mediate nuclear import independent of importin b. Journal of Biological Chemistry , Aug 6;274(32):22610-7.   

5.      CK Chan and DA Jans (1999). Synergy of importin alpha recognition and DNA binding by the yeast transcriptional activator GAL4. FEBS Letters, Nov 26;462(1-2):221-4.

6.     CK Chan, T Senden, DA Jans (2000). Supramolecular structure and nuclear targeting efficiency determine the enhancement of transfection by NLS-containing polylysine. Gene Therapy  Oct 7; (19):1690-1697. 

7.      CK Chan and DA Jans (2001). Enhancement of MSH receptor- and GAL4-mediated gene transfer by switching the nuclear import pathway. Gene Therapy.  Jan ; 8 (2):166-71. 

8.      CK Chan and DA Jans. (2002). Using nuclear targeting signals to enhance non-viral gene transfer. Journal of Immunology and Cell Biology  Apr;80(2):119-30

9.     CK Chan, EJ Brignole, W Gibson. (2002) Cytomegalovirus assemblin (pUL80a): cleavage at internal site not essential for virus growth; proteinase absent from virions. J Virology. Sep;76(17):8667-74.

10. AN Loveland, CK Chan, EJ Brignole, W Gibson (2005)  Cleavage of human cytomegalovirus protease pUL80a at internal and cryptic sites is not essential but enhances infectivity, J Virology, 79:12961-12968.

11. DK Pham, CK Chan, E Ivanova (2005) Impact of Polymeric Surfaces on Oligonucleotides Immobilization for Lab-On-A-Chip Applications; Trends in DNA Research. Nova Publishers. Ed Corey R. Woods. p 145-163;